منابع مشابه
Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana.
Sorbitol is converted to fructose in Rosaceae species by SORBITOL DEHYDROGENASE (SDH, EC 1.1.1.14), especially in sink organs. SDH has also been found in non-Rosaceae species and here we show that the protein encoded by At5g51970 in Arabidopsis thaliana (L.) Heynh. possesses the molecular characteristics of an SDH. Using a green fluorescent protein-tagged version and anti-SDH antisera, we deter...
متن کاملDetection and characterization of sorbitol dehydrogenase from apple callus tissue.
Sorbitol dehydrogenase (l-iditol:NAD(+) oxidoreductase, EC 1.1.1.14) has been detected and characterized from apple (Malus domestica cv. Granny Smith) mesocarp tissue cultures. The enzyme oxidized sorbitol, xylitol, l-arabitol, ribitol, and l-threitol in the presence of NAD. NADP could not replace NAD. Mannitol was slightly oxidized (8% of sorbitol). Other polyols that did not serve as substrat...
متن کاملA fluorimetric method for red blood cell sorbitol dehydrogenase activity.
A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the prese...
متن کاملD-sorbitol-6-phosphate dehydrogenase from Lactobacillus casei.
Several different systems for the conversion of sorbitol to hexose have been described in microorganisms. In the pseudomonads, sorbitol may be oxidized to fructose (Shaw, 1956) or to sorbose (Sebek and Randles, 1952). The latter investigators concluded that the conversion of sorbitol to fructose or sorbose did not involve phosphorylation since carbon dioxide was not liberated from bicarbonate b...
متن کاملSorbitol Dehydrogenase Activity in Diabetes Mellitus and Cataract Patients
Accumulation of sorbitol in the pathogenesis of cataract in susceptible subjects has long been an issue. Sorbitol dehydrogenase (SDH) activity in 25 diabetes mellitus (DM) patients and 15 patients having cataract was investigated using 10 subjects without diabetes or cataract as the control. Results of fasting plasma glucose assay done on the DM patients showed a mean range of 64 – 144mg/dl in ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.51.34293